chemiluminescence apparatus (Clinx Science)
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Clinx Science
chemiluminescence apparatus
Chemiluminescence Apparatus, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 3710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemiluminescence+apparatus/ChemiScope+6100+chemiluminescence+and+fluorescence+imaging+system/pmc12866308-94-55-60
Average 96 stars, based on 3710 article reviews
Chemiluminescence Apparatus, supplied by Clinx Science, used in various techniques. Bioz Stars score: 96/100, based on 3710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemiluminescence+apparatus/ChemiScope+6100+chemiluminescence+and+fluorescence+imaging+system/pmc12866308-94-55-60
Average 96 stars, based on 3710 article reviews
chemiluminescence apparatus - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: Quercetin ameliorates ox-LDL-induced cellular senescence of aortic endothelial cells and macrophages by p16/p21, p53/SERPINE1, and AMPK/mTOR pathways Article Snippet: Protein extracts were separated via 10–12% SDS-PAGE, with subsequent transference onto PVDF membranes (IPVH00010; Millipore) and closure with 5% BSA at 37 °C for 2 h. The membranes were then incubated with primary antibody solution including anti-p16 (1/5000; 10883-1-AP; Proteintech), anti-p21 (1/1000; 10355-1-AP; Proteintech), anti-SERPINE1 (1/1000; 66261-1-Ig; Proteintech), anti-Bcl2 (1/2000; 68103-1-Ig; Proteintech), anti-Beclin1 (1/2000; ab207612; Abcam), anti-GAPDH (1/5000; 60004-1-Ig; Proteintech), anti-AMPK (1/1000; ab32047; Abcam), anti-p-AMPK (1/1000; ab109402; Abcam), anti-mTOR (1/10000; ab134903; Abcam), and anti-p-mTOR (1/10000; ab109268; Abcam) at 4 °C overnight. .. Afterwards, they were washed and incubated with HRP-labeled goat anti-rabbit or anti-mouse secondary antibody solution (1/5000; ZB2301 or ZB2305; ZSGB-BIO) at 37 °C for 1 h. After washing, the ECL luminescent solution (ECL-0011; Beijing Dingguo Changsheng Biotechnology Co., LTD) was added to the membranes, and images were taken by Article Title: AGAPIR: A Novel PIWI‐Interacting RNA Enhancing Post‐Decompression Angiogenesis in Degenerative Cervical Myelopathy Article Snippet: The primary antibodies utilized included: CD31 (PECAM‐1) (D8V9E) rabbit mAb (#77 699, Cell Signaling Technology, 1:1000), von Willebrand Factor (vWF) (#GB11020, Servicebio, 1:1000), USP18 (E9K4X) (#53 229, Cell Signaling Technology, 1:1000), HIF‐1α (D1S7W) (#49 564, Cell Signaling Technology, 1:1000), VHL rabbit pAb (#A0377, ABclonal, Wuhan, China, 1:1000), GAPDH rabbit polyclonal antibody (#ABL1012, Abbkine, Wuhan, China, 1:1000), and α‐tubulin monoclonal antibody (#ABL1080, Abbkine, 1:1000). .. The following day, the membranes were incubated with horseradish peroxidase (HRP)‐conjugated anti‐rabbit (#SSA004, Sino Biological, Beijing, China, 1:3000) or anti‐mouse (#SSA007, SinoBiological, 1:3000) secondary antibodies at room temperature for 1 h. All membranes were developed using Western Bright ECL HRP substrate (#G2161, Servicebio), and signals were detected using a Article Title: STUB1-mediated PGC-1α ubiquitination promotes postoperative cognitive dysfunction in aged mice via modulating autophagy–lysosome machinery Article Snippet: The membrane was rinsed by TBST (3 times × 5 min), and then slowly shaken with 5% BSA at 37 °C for 2 h. Incubation with primary antibodies of STUB1 (1:1000, 68407-1-Ig, Proteintech), PGC-1α (1:1000, 66369-1-Ig, Proteintech), TFEB (1:1000, 13372-1-AP, Proteintech), PINK1 (1:1000, ab300623, Abcam), PARKIN (1:1000, 14060-1-AP, Proteintech), LAMP-1 (1:1000, 21997-1-AP, Proteintech), Cathepsin D (1:1000, 21327-1-AP, Proteintech), Caspase3 (1:1000, 19677-1-AP, Proteintech), ubiquitin (1:1000, 10201-2-AP, Proteintech), and GAPDH (1:5000, 60004-1-Ig, Proteintech) was conducted at 4 °C overnight. .. After rinsing by TBST (3 times × 1 min), incubation with HRP-labeled goat anti-rabbit or anti-mouse IgG (1:5000, ZB2301 or ZB2305, ZSGB-BIO) was conducted at 37 °C for 1 h. After rinsing with TBST (3 times × 5 min), ECL luminescent solution (ECL-03-250, 7Sea Biotech, China) was added, and images were acquired via an integrated Article Title: AGAPIR: A Novel PIWI-Interacting RNA Enhancing Post-Decompression Angiogenesis in Degenerative Cervical Myelopathy. Article Snippet: The primary antibodies utilized included: CD31 (PECAM-1) (D8V9E) rabbit mAb (#77 699, Cell Signaling Technology, 1:1000), von Willebrand Factor (vWF) (#GB11020, Servicebio, 1:1000), USP18 (E9K4X) (#53 229, Cell Signaling Technology, 1:1000), HIF-1α (D1S7W) (#49 564, Cell Signaling Technology, 1:1000), VHL rabbit pAb (#A0377, ABclonal,Wuhan, China, 1:1000), GAPDH rabbit polyclonal antibody (#ABL1012, Abbkine, Wuhan, China, 1:1000), and α- tubulin monoclonal antibody (#ABL1080, Abbkine, 1:1000). .. The following day, the membranes were incubated with horseradish peroxidase (HRP)conjugated anti-rabbit (#SSA004, Sino Biological, Beijing, China, 1:3000) or anti-mouse (#SSA007, SinoBiological, 1:3000) secondary antibodies at room temperature for 1 h. All membranes were developed using Western Bright ECL HRP substrate (#G2161, Servicebio), and signals were detected using a Western Blot:Article Title: AGAPIR: A Novel PIWI‐Interacting RNA Enhancing Post‐Decompression Angiogenesis in Degenerative Cervical Myelopathy Article Snippet: The primary antibodies utilized included: CD31 (PECAM‐1) (D8V9E) rabbit mAb (#77 699, Cell Signaling Technology, 1:1000), von Willebrand Factor (vWF) (#GB11020, Servicebio, 1:1000), USP18 (E9K4X) (#53 229, Cell Signaling Technology, 1:1000), HIF‐1α (D1S7W) (#49 564, Cell Signaling Technology, 1:1000), VHL rabbit pAb (#A0377, ABclonal, Wuhan, China, 1:1000), GAPDH rabbit polyclonal antibody (#ABL1012, Abbkine, Wuhan, China, 1:1000), and α‐tubulin monoclonal antibody (#ABL1080, Abbkine, 1:1000). .. The following day, the membranes were incubated with horseradish peroxidase (HRP)‐conjugated anti‐rabbit (#SSA004, Sino Biological, Beijing, China, 1:3000) or anti‐mouse (#SSA007, SinoBiological, 1:3000) secondary antibodies at room temperature for 1 h. All membranes were developed using Western Bright ECL HRP substrate (#G2161, Servicebio), and signals were detected using a Article Title: AGAPIR: A Novel PIWI-Interacting RNA Enhancing Post-Decompression Angiogenesis in Degenerative Cervical Myelopathy. Article Snippet: The primary antibodies utilized included: CD31 (PECAM-1) (D8V9E) rabbit mAb (#77 699, Cell Signaling Technology, 1:1000), von Willebrand Factor (vWF) (#GB11020, Servicebio, 1:1000), USP18 (E9K4X) (#53 229, Cell Signaling Technology, 1:1000), HIF-1α (D1S7W) (#49 564, Cell Signaling Technology, 1:1000), VHL rabbit pAb (#A0377, ABclonal,Wuhan, China, 1:1000), GAPDH rabbit polyclonal antibody (#ABL1012, Abbkine, Wuhan, China, 1:1000), and α- tubulin monoclonal antibody (#ABL1080, Abbkine, 1:1000). .. The following day, the membranes were incubated with horseradish peroxidase (HRP)conjugated anti-rabbit (#SSA004, Sino Biological, Beijing, China, 1:3000) or anti-mouse (#SSA007, SinoBiological, 1:3000) secondary antibodies at room temperature for 1 h. All membranes were developed using Western Bright ECL HRP substrate (#G2161, Servicebio), and signals were detected using a |